[仪器介绍]

ÄKTA avant 是用于方法探索和纯化工艺优化的全智全能的液相色谱系统，现有两个型号。两款系统具有不同的流速 压力参数，但具有相同的硬件装置。ÄKTA avant 25 系统单泵最大设计流速为25 ml/ min ，用于填料筛选，方法优化和微克级以至百毫克级样品分析和制备。ÄKTA avant 150 系统单泵最大设计流速为150 ml/min，可用于填料筛选方法优化和工艺放大， 以及微克至数十克级别生物活性物质的分析和制备。 UNICORN ™ 控制软件被用于控制自动化系统ÄKTA avant ，以提高纯化效率。

ÄKTA avant 是一种设计用于方法探索、纯化优化和制备放大的色谱系统。ÄKTA avant 为快速、高质量的蛋白质分离，并同时为灵活性和工艺可靠性提供一种完整的解决方案。

[关键性能和优点]

1．整合在UNICORN 软件中的实验设计（DoE）工具，可通过在更少的系统性设计的实验，捕捉更准确的信息，从而节约时间和成本 ；

2．可以识别每根色谱柱及其运行历史数据，提供可追溯性，保障凝胶操作的可靠性 ；

3。集成半导体制冷的组分收集器，可以保护纯化的样品活性 ；

4．BufferPro自动在线缓冲液配制功能减少了用于缓冲液混合和手动滴定所需要的时间，提高您的工作效率和产出 ；

5．UNICORN 软件采用可视化程序编辑界面用于直观和灵活的方法编辑，并能够在不同纯化规模的ÄKTA avant上进行方法转换，直观而轻松完成制备规模的线性放大。

[设备用途] 快速纯化多种生物活性物质，如蛋白质、多糖、肽类、寡核苷酸、基因疫苗及病毒等。其自动化的配置满足从工艺开发、优化到中试的需求，DOE结果与分布图提供良好的验证支持。

[样品和缓冲液要求] 样品必须过滤，缓冲液必须过滤脱气（在操作培训教材中的实验部分有介绍）

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